Loading Waters/Micromass RAW data

PEAKS handles Waters/Micromass mass spectrometry data in it's .RAW folder, as long as you have MassLynx installed on the same computer. PEAKS uses a converter called wolf.exe to convert the RAW data to an mzXML file behind the scenes, then seamlessly loads the mzXML file. Conversion can take some time. The conversion utilities used are:

• The sashimi project's official release wolf.exe for Masslynx 4.0 and 4.1 -- untested by BSI but this is the newest version.
• On March 12th, 2007 BSI compiled wolf.exe for Masslynx 4.1, since there was no version available for Masslynx 4.1 at the time. This is the version that ships with PEAKS.
• The last version of wolf.exe for Masslynx 4.0 that was tested by BSI is provided here for download, in case users have a problem with the newer version listed above.

Click above to download, save it in your peaks program folder (usually c:\program files\peaks studio\).

Once MassLynx is installed, to import RAW data into PEAKS you just have to choose File -> ImportRAW -> Waters (.RAW folder) then browse to the file on your system.

If at all possible, be sure to use non-preprocessed data with the PEAKS software. PEAKS results will be better when deconvolution and centroiding are done by our software only. Be sure to check the "Centroid and deconvolute for analysis" option in the "Protein Identification" window when you are analyzing your data with PEAKS.

If any errors or inaccuracies are noted from this page please contact us.